Granulocyte-macrophage colony stimulating factor exacerbates collagen induced arthritis in mice.

Blockade of collagen-induced arthritis post-onset by antibody to granulocyte-macrophage colony-stimulating factor (GM-CSF): requirement for GM-CSF in the effector phase of disease

There is mounting evidence for a role of the growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF) in inflammatory disease, including arthritis. In the present study, we examined the effectiveness of treatment of collagen-induced arthritis (CIA) with a neutralizing mAb to GM-CSF. DBA/1 mice were immunized for the development of CIA and treated at different times, and with diff...

Effector mechanisms of interleukin-17 in collagen-induced arthritis in the absence of interferon-γ and counteraction by interferon-γ

INTRODUCTION Interleukin (IL)-17 is a pro-inflammatory cytokine in rheumatoid arthritis (RA) and collagen-induced arthritis (CIA). Since interferon (IFN)-gamma inhibits Th17 cell development, IFN-gamma receptor knockout (IFN-gammaR KO) mice develop CIA more readily. We took advantage of this model to analyse the mechanisms of action of IL-17 in arthritis. The role of IFN-gamma on the effector m...

Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli

Protection from collagen-induced arthritis in granulocyte-macrophage colony-stimulating factor-deficient mice.

The involvement of granulocyte-macrophage CSF (GM-CSF) in collagen-induced arthritis (CIA) was examined using GM-CSF-deficient mice. Although CIA is generally considered to be restricted to mice of the H-2q or H-2r haplotypes, we examined the role of GM-CSF in the CIA model using GM-CSF-deficient (-/-) and wild-type (+/+) mice on a C57BL/6 (H-2b) background. Mice were immunized by intradermal i...

The Anti-Rheumatoid Arthritis Effects of Rhododendron Molle Roots on Collagen-Induced Arthritis in Rats

Abbreviations: RA: Rheumatoid Arthritis; CIA: Collagen-Induced Arthritis; IL-1β: Interleukin-1β; IL-6: Interleukin-6; IL-17A: Interleukin17A; IFN-γ: Interferon-γ; TNF-α: Tumor; Necrosis Factor-α; GM-CSF: Granulocyte-Macrophage Colony-Stimulating Factor; IL-8: Interleukin-8; MTX: Methotrexate; CSA: Cyclosporine; AZA: Azathioprine; LEF: Leflunomide; CFA: Complete Freund’s Adjuvant; ELISA: Enzyme ...